Development of an inoculum with different substrates through solid state fermentation [Desarrollo de un inoculo con diferentes sustratos mediante fermentaci�n s�lida sumergida]

The aim of this study was to develop an inoculum with four different substrates, these were evaluated with three commercial yeasts A, B and C of the genus Saccharomyces cerevisiae yeast and apple know, D, of the genus Kluyveromyces lactis, through its development aerobic conditions using oxygen in each liquid culture medium. During the fermentation 3 samples, were taken for treatment at different times (0, 2, 4, 8, 16, 32, 64 and 128 h), pH, temperature, carbohydrates, yeast counts and ammonia nitrogen were determined. A randomized statistical design was used with 4x4 factorial arrangements in plots using the procedure (Proc Mixed) program (SAS, 2004). The pH showed a similar pattern for all substrates with quadratic effect (P <0.01), and also showed an interaction (P <0.01) treatment by the quadratic effect of time, indicating that this increase was different between treatments. Temperature was increased (P <0.01) of the h128 h0 in all treatments, then declined (P <0.01) to stay close to the incubation temperature from h8. Ammonia nitrogen, difference was observed (P <0.01) between treatments and yeast. Yeast Count, an effect was observed (P