Fourier transform infrared spectroscopy in kidney cancer and healthy tissue differentiation

Renal cancer is one of the most common malignancies in human and it is also one of the most leading causes of cancer-related death. Most of the methods used for this purpose nowadays are based on histopathology, but is needed time saving method we could use during the surgery. Recently, Fourier transform infrared (FTIR) spectrometry is considered to develop into a new method for cancer diagnosis, which is non-destructive and time-consuming procedure, but until know there are lack of research to use this method as a main of cancer diagnosis. The goal of this study was to investigate intercellular fluid of cancerous and healthy tissues by means of FTIR method. To achieve this goal an acquaitaince with scientific literature of this field and measurments and analysis of healthy and cancerous tissues was done. The results: there were measured 78 IR absorptions spectra of intersellular fluid of tissues in the spectral region from 1800 to 950 cm-1 and where were found six spectral cancer markers: lipids vibrations around 1740 cm-1, the amide III band about 1308 cm-1, nucleic acids and phospholipids about 1240 cm-1 and 1082 cm-1, RNA ribose around 1158 cm-1 and glycogen spectral band about 1030 cm-1. Intensities of all aforementioned bands relative to amide I band are higher in healthy tissues and thus concentrations of these molecules in cancerous tissue are increased. ATR-FTIR spectrometry may be developed into a rapid promising method for judging surgical resection margins of cancer