Analysis of evaluation of haemolysis influence on blood coagulation test's results

Analysis of Evaluation of Haemolysis Influence on Blood Coagulation Test's Results Recently, a lot more attention is dedicated to ensure the quality of laboratory testing. The quality, accuracy and reliability of investigations carried out in the laboratory depend on many factors. It was found that as much as 70 percent of errors that are discovered in the laboratory occur in pre-analytical phase. However, the main reason for specimen rejection is hemolysed specimens. Although hemolysis influence of various analytes has been known, the lack of thorough research influences different degrees of hemolysis usually performed in blood coagulation tests. For this reason, it was decided to investigate the influence of hemolysis degrees usually conducted from specimens of blood coagulation- SPA, APTT, fibrinogen and d-dimer. During the study, a total of 160 analysed specimens were caused by varying levels of (1+, 2+, 3+, 4+) hemolysis using lysing solution. The study results showed that, with varying degrees of hemolysis (1+, 2+, 3+, 4+) SPA activity and fibrinogen concentration in blood plasma decreases significantly whereas dimer concentration in plasma is increasing. Statistically significant differences were obtained 3+ and 4+ of hemolysis specimen. Also, our research has shown that varying levels (1+, 2+, 3+, 4+) of hemolysis does not affect the APTT activity in plasma. The obtained results prove that hemolysis affects laboratory research of blood coagulation therefore it is essential to consider even slightly hemolysed samples with a possibility of rejection