Ekspresija proteina sindekan-1 i HSP-70 u tkivu pacijenata s teškim osteoartritisom kuka

Objectives: The purpose of this study is to investigate the expression of Syndecan-1 and HSP-70 in synovial, chondral and subchondral tissue in the hip of human patients with osteoarthritis according to their disease severity. Materials and methods: Total hip arthroplasty with tissue extraction from the weightbearing area of the pathologic femoral head was performed on all patients. Patients were divided into two groups according to their disease severity defined by their Kellgran Lawrence, Harris Hip and Oxford Hip Score: control (femoral neck fracture) and severe Osteoarthritis (OA). Chondral, subchondral and synovial tissues were analyzed with raised antibodies against Syndecan-1 and HSP-70. Results: Immunohistochemical staining with Syndecan-1 and HSP 70 antibodies revealed positive expression of both markers. It was established that the chondral, subchondral and synovial tissue in patients with severe OA contained abundant positive cells with a mean positive cell count of 120±16 and 142±23 for Syndecan-1 and 120±24 and 136±28 for HSP-70, respectively. On the contrary, patients without OA had only minimal positivity with a mean positive cell count of 30±4 and 34±16 for Syndecan-1 and 30±7 and 33±22 for HSP-70, respectively. Synovial tissue could not be identified enough for presentation. However, it followed the trend of chondral and subchondral tissue. It was established that there was a statistical significance of both Syndecan-1 and HSP-70 with p < 0.05 in cartilage and p < 0.001 in subchondral tissue between control and severe OA. Conclusions: Patients with severe osteoarthritis had increased staining of Syndecan-1 and HSP-70 in the cartilage and subchondral tissue of the hip compared to patients who underwent total hip replacement for a femoral neck fracture. Increased expression implies that Syndecan-1 and HSP-70 could play a significant role in the complex pathophysiology of osteoarthritis but further research is needed in order to make these findings applicable for clinical treatments.Ciljevi: Svrha ovog istraživanja bila je usporediti razinu ekspresije proteina sindekan-1 i HSP-70 u sinoviji, hrskavici i subhondralnoj kosti pacijenata s teškim osteoartritisom (OA) kuka i pacijenata bez osteoartritisa. Materijali i metode: Sukladno težini pripadajuće bolesti, određenoj prema Kellgren-Lawrence, Harris Hip i Oxford Hip klasifikaciji, ispitanici su podijeljeni u dvije grupe: eksperimentalna grupa (teški OA) i kontrolna grupa (prijelom vrata femura). Svim ispitanicima učinjena je totalna artroplastika kuka s ekstrakcijom patološki oštećenog tkiva. Ekstrahirano tkivo sinovije, hrskavice i subhondralne kosti je imunohistokemijski analizirano pri čemu su korištena protutijela na sindekan-1 i HSP-70. Rezultati: Imunohistokemijska analiza s protutijelima na sindekan-1 i HSP-70 pokazala je pojačanu ekspresija obaju proteina u hrskavici (H) i subhondralnoj kosti (SHK) u eksperimentalnoj grupi u odnosu na kontrolnu. Zbog nedovoljno ekstrahiranog sinovijalnog tkiva, na sinoviji nije bilo moguće pouzdano usporediti grupe, no dojam je da bi rezultat bio sukladan onom u H i SHK. U pacijenata s teškim OA, u H i SHK, zabilježeno je obilje ''pozitivnih'' stanica sa srednjom vrijednošću od 120±16 i 142±23 za sindekan-1 te 120±24 i 136±28 za HSP-70. U pacijenata bez osteoartritisa zabilježena je blaga ekspresija ispitivanih proteina u H i SHK sa srednjom vrijednošću ''pozitivnih stanica'' od 30±4 i 34±16 za sindekan-1 te 30±7 i 33±22 za HSP-70. Utvrđena razlika u ekspresiji navedenih proteina statistički je značajna s razinom značajnosti p<0,05 za hrskavicu i p