Expression of kit ligand and amount of follicles as features of folliculogenesis disorder on rat (rattus novergicus) strain wistar with cisplatin

Kit Ligand (KL), one of the first growth factors in ovarian follicle, plays a key role in mammalian oogenesis and folliculogenesis. KL protein and mRNA expression is detected in the granulosa cells of follicles at all stages of development. Cisplatin is one of the first generation platinium compounds and is commonly used for treatment of ovarian, breast, testicular, and bladder cancers.The objective of this study was to prove the lower expression of kit ligand and amount of follicles, and the association between the expression of kit ligand and the amount of follicles, on rat (Rattus novergicus) strain wistar with cisplatin. This study was conducted at Laboratory of Experimental Animal and Veterinary Patology, Faculty of Veterinary, Airlangga University, Surabaya, May-July 2011. The research was performed by randomized post test only control group design. The research samples were Rat (Rattus novergicus) strain wistar which was admitted into inclusion criteria. At the diestrus period, the control group was injected by NaCl 0.9% IP, and the cisplatin group was injected by cisplatin 5 mg/kg w IP. The rat were slaughtered at the 7th day of estrus period on the control group, and at the diestrus period on the cisplatin group. Oophorectomy was performed, continued with immuno-histochemistry staining and hematoxylin eosin for examination on expression of kit ligand and amount of follicles. We achieved no association among the expression of kit ligand and the amounts of primordial, primary, secondary, and tertiary follicles (p=0.945, p=0.180, p=0.590, p=0.753) on the rat with cisplatin. In conclusion, In rat with cisplatin, the expresssion of kit ligand, the amount of primordial, primary, secondary, and tertiary follicles are low. (MOG 2011;19:102-108