Cell Culture: Future Prospect

The cell culture was initially by Ross Harrison in 1907 who demonstrated the frog embryo growth in vitro. This technology showed the important role since the specific individual cells and the first continuous cell lines were developed and the cells were not only kept alive by cryopreservation technology but also they grew as they would do in vivo after thawing and reculturing. The specific requirements of the differences cell types like local micro-environment, local growth factors, extra cellular matrix, cell- cell interactions, circulating proteins, cytokines and hormones were being developed for the growth of specific cell types desired. The advances in cell culture techniques enable researchers to create at least partially model complex interactions, drug testing and investigate toxic effects, further more incorporating cells from several different tissues in the culture offer the opportunity to create the interactions among separate biological subsystems in vitro. The major benefit of cell culture technology utilisation is not only greatly felt in the biomedical, production and reproduction areas but also in the biopharmaceutical development. The future prospective of cell culture technology among others perfect cell culture system for the preparation of autologous cell lines, the processing of xenogenic cell lines for cellular implants, gene therapy, artificial organ, and three-dimensional cell cultures development. Key words: Cell culture, in vitro, spesific requirement, perfect cell culture syste