Protective Effect of Propolis Extract in Kidney Male Mice (Mus musculus) Induced by Lead Acetate

The aim of this research was to investigate the protective effect of propolis extract from Apis mellifera, on histopathological changes of the kidney induced by lead acetate in mice (Mus musculus). 25 male mice were randomly divided into five groups and administered orally with different treatments for 35 days. The treatment were Group C- (CMC-Na 1.5% and Tween 80 0.5% continued with aquadest one hour after the first administration), Group C+ (CMC-Na 1.5% and Tween 80 0.5% and continued with 20 mg/kg bw of lead acetate), and treatment group T1, T2, T3 (200, 400, 800 mg/kg bw propolis extract and continued with 20 mg/kg bw of lead acetate). After treatments, tissues were processed, and histopathological evaluation were examined using Arshad Scoring method. The result showed necrosis and tubular cast in the treatment group of T3 (1.44±0.09a and 1.04±0.43ab) have no significant difference with group of C- (1.24±0.33a and 0.96±026a). The mean value of necrosis and tubular cast in group T3 is the lowest, while the mean value of hydropic degeneration is the highest. It indicates the reversible cell injury. Thus, the effective dose of propolis extract used in this research is 800 mg/kg bw. Experimental article (J Int Dent Med Res 2017; 10(3): pp. 1060-1065) Keywords: Propolis, lead, free radicals, reactive oxygen species, kidne