Pengaruh Berbagai Konsentrasi Krioprotektan Propanediol pada Proses Vitrifikasi Terhadap Viabilitas Embrio Mencit Pasca Thawing

This experiment was doing to know the effects concentration of cryoprotectant at vitrification on viability of mice’s embryos. Embryos were exposed to a different concentration of propanediol cryoprotectant, that is concentration 10%, 20% and 30%. Embryos were kept in mini straw and then plunged into liquid nitrogen rapidly. After 2 week, the frozen embryos were thawed in a 30°C water bath and be washed twice in 0,5 M Sucrose. Viability of embryos was examined used inverted microscope. The result showed there have a significant differences (p < 0,05) for embryos survival among treatment of propanediol 10% (46,95 %), propanediol 20%(51,73 %) and propanediol 30% (54,94 %). A various concentration of cryoprotectant was give a different result atembryos viability. The conclusion of this experiment there is a real difference (p < 0,05) at a various concentration of propanediol cryoprotectant with a good result give in propanediol 30% (54,94 %