PENGARUH KONSENTRASI POLIMER NATRIUM ALGINAT DENGAN PENYAMBUNG SILANG CaCl2 1 MOLAR (M) TERHADAP KARAKTERISTIK MIKROSFER ERITROPOETIN-ALGINAT (Dibuat Menggunakan Metode Gelasi Ionotropik Teknik Aerosolisasi)

The aim of this research was to determine the effect of sodium alginate concentration on the characteristics (morphology, particle size, swelling index, and yield) of erythropoietin-Ca alginate microspheres using gelation ionotropic method with aerosolization technique. The amount of erythropoietin added was of 5000 IU (70 μl) into 1% w/v, 2%w/v and 3% w/v of alginate solution and CaCl2 1M at stirring speed 1000 rpm in 30 minutes. Microsphere formed was resuspended into the solution of maltodextrin (as lyoprotectant) and was dried using freeze dryer. Microspheres evaluation included FT-IR, DTA, investigation of moisture content, morphology using SEM, particle size distribution using optical microscopy, determination of swelling index, and yield. Microsphere had spherical form, smooth surface and the size were below 5 μm. Determination of swelling index did by two methods, they are calculation of mass differentiation and calculation of size differentiation by time of 24th and 30th. Result of examination from swelling index in F1, F2, F3 are 0.58, 1.25, 1.43 (time of 24th); 0.78, 1.78, 2.16 (time of 30th) 0.50, 1.15, 1.32 (size method with time of 24th), and 0.65, 1.80, 1.98 (size method with time of 30th). The result of yield formula F1, F2, F3 were 75.55 ± 0,350; 77,84 ± 0,290; 86,65 ± 0,191. Result of analysis show that the increasing of alginate concentration cause the increasing of particle size, swelling index and also yield