KARAKTERISASI MIKROSFER NISIN DENGAN MATRIKS NATRIUM ALGINAT-GELATIN (2,5 : 0,5)% (Dibuat dengan Metode Ionotropic Gelation Teknik Aerosolisasi)

The aim of this research is to characterize nisin microsphere were made by ionotropic gelation method aerosolization technique with sodium alginate - gelatine (2.5 : 0.5)% as matrix polymer and calcium chloride 1.5 M as the cross linker. The verification of nisin microsphere was determined using FTIR and X-Ray Diffraction. The interaction between polymer and nisin was confirmed by the changes in the intensity and wave number on the FT-IR spectra of nisin microsphere. X-Ray Diffraction of nisin microsphere showed amorf characteristic. The evaluation of nisin microsphere was determined by yield value and percent moisture content. Nisin microsphere has yield value 60.81% and has moisture content 8.90 ± 0.15(%). Nisin microsphere is spherical with smooth surface structure as displayed by scanning electron microscope (SEM). Nisin microsphere has mean particle diameter 5.14 + 0.40(μm). Mean particle diameter and particle size distribution were determined by Biological Microscope Model XSZ-107 Series. Swelling characteristic was determined using gravimetric swelling index procedure and the maximum swelling index is 1141± 34.58(%) achieved within 5 hr. Entrapment efficiency and drug loading was determined using Spectra/Por® molecularporous membrane tubing (Dialysis Membrane) MWCO: 6-4 kD. Assay of nisin was determined using Spectrophotometer UV 1800 Shimadzu. Nisin microsphere has entrapment efficiency 35.64 ± 0.93 (%) and drug loading 4.50 ± 0.12(%)