PROFIL RESISTENSI ISOLAT BAKTERI Escherichia coli DARI SUSU SAPI SEGAR BEBERAPA PETERNAKAN SAPI PERAH DI SURABAYA TERHADAP BEBERAPA ANTIBIOTIK

The objective of this study was to isolate and identify in bacteria of the species Escherichia coli, and to show profile resistance antibiotics Ampicillin, Gentamicin, Oxytetracycline, Trimethoprim, and Chloramphenicol of bacteria Escherichia coli from raw milk on farms dairy cattle in Surabaya. A total of 40 raw milk samples were collected from 4 different dairy farms in Surabaya (Pogot, Kaliwaron, Wonocolo, and Bendul Merisi). Milk samples taken during the morning milking at 04.00-06.00 WIB. Preparation of the isolation and identification was confirmed by Brilliant Green Bile Broth, Eosin Methylene Blue Agar, and indole test using media Peptone Water 1%. Escherichia coli isolates were tested using the test sensitivity to antibiotics by Kirby-Bauer method. The results showed that isolates of Escherichia coli from raw milk at dairy farm in Surabaya, resistant to antibiotics ampicillin by 100%, 60% were resistant to oxytetracycline and trimethoprim, 90% were resistant to chloramphenicol, but antibiotic gentamicin still sensitive by 100%. Based on the result, we can conclude that there are Escherichia coli bacteria that resistance to antibiotics ampicillin, oxytetracycline, trimethoprim, and chloramphenicol. This is because the use of antibiotics that are not rasionale, and in the long term can lead the emergence of antibiotic resistance