PENGARUH PEMBERIAN EKSTRAK KULIT MANGGIS (Garcinia mangostana Linn.) TERHADAP EKSPRESI TGFβ-1 PADA KULTUR SEL HUMAN GINGIVAL FIBROBLAST (in vitro study)

Background: Tooth extraction caused an injury to the tooth socket and the process of wound healing which has four phases: hemostasis, inflammation, proliferation, and remodeling. Inflammation phase will stimulate the migration and the expression of TGFβ-1 which can accelerate the proliferation of fibroblast. Mangosteen peel extract contain saponin that has activity as an anti-inflamatory that can help the process of fibroblast proliferation. Purpose: The aim of this study was to investigate the effect of the application of mangosteen peel extract towards TGFβ-1 expression on human gingival fibroblast cell culture. Methods: Human gingival fibroblast cell culture divide on 6 well plates for each category: control 24 and 48 hours, the treatment of 24 and 48 hours. The treatment groups were given mangosteen peel extract at concentration 800μg/ml. then the extraction of the RNA was done and the RNA were processed by PCR assay. Results: Group of the treatment 24 and 48 hours show a brighter band than the control group 24 and 48 hours. The score of 24 hours group not as significant as the 48 hours group. Conclusion: The application of the mangosteen peel extract can increase the expression of TGFβ-1 on human gingival fibroblast cell culture. Keywords: Mangosteen peel extract, growth factor, TGFβ-1,PCR assay, human gingival fibroblast