ANALISIS RESPON JARINGAN PASCA PENANAMAN SUBCUTAN DEMINERALIZED FREEZE DRIED BOVINE CORTICAL BONE MEMBRANE (DFDBCBM) Penelitian Experimental Labolatorik in vivo pada Tikus Strain Wistar

Background : The procedures of making collagen membrane for Guided Bone Regeneration (GBR) requires technology and high cost. It makes the price of bioabsorbable and biodegradable membrane products in the market is relatively expensive. Because of that reason, it is needed innovation manufacture of collagen membrane which match with the criteria of GBR membrane but the price is affordable by the people of Indonesia. Purpose: This research is expected to provide information on how the tissue response after implantation Demineralized Freeze-Dried Bovine Cortical Bone (DFDBCB) Membrane in the tissue and about the potential application of Demineralized Freeze-Dried Bovine Cortical Bone (DFDBCB) Membrane as a tissue barrier in Guided Bone Regeneration (GBR) procedures. Methods: This study using strain Wistar rats which is available at the Biochemistry department, Faculty of Medicine UNAIR, DFDBCB membrane that prepared by dr. Soetomo hospital and membrane Jason Botiss as controls. Consider the probability of homogeneity data that want to be collected, we used 5 samples for each treatment. The total number of samples are 40 samples. This is experimental research with post test only group design. This study evaluated the thickness of the capsule, the quality of the capsule fibrous, and the quality of the interface on the dorsum of the rat strain Wistar after implanting of Demineralized Freeze-Dried Bovine Cortical Bone (DFDBCB) Membrane on a medium in-vivo at days 7, 14, 21, and 28. Result: The results from this experiment is there is no difference in the quantity of fibroblasts capsule between the DFDBCBM with pericardium membrane group. There is no difference in quality of the capsule fibrous between pericardium membrane group and DFDBCBM. And there is no difference in the quality of the interface tissue between the DFDBCBM with pericardium membrane group. Conclusion: Demineralized Freeze Dried Bovine Cortical Bone Membrane is equal with the pericardium membrane, which is available in the market these days. Key words: Collagen Membrane, Pericardium Membrane, DFDBCBM membrane, Guided Bone Regeneration