Peningkatan Kolagen Akibat Induksi Gel Kombinasi Spirulina dan Kitosan Pada Soket Bekas Pencabutan Gigi Marmut (Cavia cobaya)

Background: Spirulina contains a variety of nutrition, such as C-phycocyanin, cytokines, SOD(Super Oxide Dismutase), beta carotene , vitamin B, vitamin E, zinc, protein, amino acids, and other trace elements as well as natural phytochemicals that can accelerate wound healing process on tooth extraction socket. On the other hand, chitosan contains N acetyl-D glucosamine which polymer structure similar to hyaluronic acid classified into glycosaminoglycan group (GAGs), which is extracellular matrix molecule that is important for wound healing. Objective: This research is aimed to study the effects of combination of spirulina and chitosan gel applied topically on tooth extraction socket. Method: This research used 28 cavia cobayas and divided into 4 groups,then the lower left incisor of those in each group was extracted. In control group, tooth extraction socket was then given 3 % CMC Na topically, in group 1 was given 3 % CMC Na mixed with 3 % spirulina and chitosan 200 mg, in group 2 was given 3 % CMC Na mixed with 6 % spirulina and chitosan 200 mg, in group 3 was given 3 % CMC Na mixed with 12 % spirulina and chitosan 200 mg. After 30 days, they were obtained histopathological preparations. And then, the amount of collagen was calculated and analyzed statistically by using Komolgorov - Smirnof test, one- way ANOVA test, and post hoc Tuckey HSD type test. Result: There was significant difference of the amount of collagen between the control group and the third treatment groups with Sig > 0.05. Conclusion: the application of 3 % CMC Na gel mixed with the combination of spirulina and 12 % chitosan 200 mg in the socket after tooth extraction can increase the amount of collagen