Natural Phytoestrogen Contents in Several Fruits; the Future Replacement Hormone Therapy in Menopause Women

Phytoestrogen is moleculaily almost similar with and act same as estrogen and found lot in several fruits and leafs mainly in tropical countries. However, its quantitative the molecular contents is not yet known exactly. Menopause and andropause people needs substitution as a replacement therapy of sex hormone, because they have declined the hormone significantly so impacted disturbance of several organs dysfunction. The objective of this study was to analyze the estrogen content in extract pegaga, green clover leafs, tomato and papaya fruit, so then a positive impact felt in certain community . The samples were collected using purposive sampling with 10 times replicate in four different groups there were pegaga, green clover leafs, tomato and papaya fruit. All these groups divided into 2 subgroups based on process or subspecies. All samples were made infuse/infusa 1:4) w/v) then extracted after was spinning 1000 xg for l5 mnts, with 1:5 petroleum ether)v/v). After evaporated each extract then kept dry-frozen -20oC until analysis to be performed. Solid phase Radioimmunoassay technique was used to identifu the estrogen content. The lowest estrogen level presented in fresh pegaga leafs extract (Mean+Sd) was 47.9+5.5 pg/g and twice increasing the level in dry leafs extract was 96.L + 11.2pg/g. Meanwhile the estrogen level in fresh green clover leafs extract was 538.0+30.5 pg/g more than ten times higher if compared to fresh pegaga level, but more less lower twice the estrogen level it was compared to dry green clover leafs extract, there was 1068.0+97.2 pg/g. ln fruit group appear fibrin part of tomato has more less the same estrogen content with Thai papaya subspecies there were 1037.0+37.7 pg/g and 1175.0+ 67.7p g/g respectively