PENGARUH PENAMBAHAN Lactobacillus plantarum TERHADAP AKTIVITAS ANTIBAKTERI EKSTRAK AIR TEH HIJAU (Camellia sinensis L. Kuntze)

The effects of adding Lactobacillus plantarum to green tea water extract (Camellia sinensis) on increasing of its antibacterial activity Safarini Marwah Green tea water extract (GTWE) is rich in polyphenols that can be used as antibacterial. However, polyphenols are not completely absorbed and Lactobacillus plantarum, act as probiotic, can metabolized it and has synergistic effect of antibacterial activity with GTWE. The aims of this study was to assess the effect of Lactobacillus plantarum addition on antibacterial activity of GTWE. The green tea leaf from PTPN XII Lawang was used as sample of this experiment. MICs and co-culture of combination GTWE and Lactobacillus plantarum FNCC-0027 were measured. In co-culture studies, antibacterial activity the combination of Lactobacillus plantarum FNCC-0027 and GTWE was observed against both Staphylococcus aureus and Escherichia coli. The results showed that the Lactobacillus plantarum growth was not affected by GTWE, and antibacterial activity the combination of Lactobacillus plantarum FNCC-0027 and GTWE was significantly increase. We found that MICs of GTWE against Staphylococcus aureus (3200 μg.mL-1) were considerably lower than those against Escherichia coli (6300 μg.mL-1). The diameter inhibitory zone of GTWE in combination with Lactobacillus plantarum FNCC-0027 is longer than Lactobacillus plantarum or GTWE used separately. As probiotics and the green tea are derived from natural products, treatment with these agent may represent alternatives to antibacterial therapy. Keywords : Green tea water extract, Lactobacillus plantarum, antibacterial activit