ISOLASI SENYAWA RUTIN DARI TUMBUHAN Phyllanthus niruri LINN

Rutin is one of the substance which occur in Phy//antus niruri L. Which useful as antioxidant and antiinflarnacy. Substances have isolated with vacuum liquid chromatography using Choroform.: methanol with many comparation as eluen in extract separation. Research shown that identically substance with rutin is occur in fraction 18 -21 with weight 427 mg. The substances from fraction 18-21 were purified with TLC preparatif and one spot was gained in second TLC preparatif. And purified with Open coloumn Chromatography as alternative method. Qualitative analysis using TLC scanner acquired 265 nm and 365 nm as the identically A maximum with rutin standard., and the match factor is 997,329. From the open coloumn chromatography obtain 265 nm and 360 nm as the A maximum. And the match metor is 997,351