THE EFFECT OF Mimosa pudica EXTRACT TOWARDS THE PRODUCTION OF ANTIVENOM IMMUNOGLOBULIN G (IgG) IN RATS (Rattus norvegicus) IMMUNIZED WITH JAVAN SPITTING COBRA (Naja sputatrix) TOXOID

In the field of herbal medicine, Mimosa pudica has been discovered to possess many beneficial properties, including wound-healing, antimicrobial, analgesic, antivenom, and immunostimmulant activities. The aim of this research is to explore the capabilities of Mimosa pudica in stimulating the production of antivenom immunoglobulins in rats immunized with toxoid derived from Naja sputatrix. The subjects were grouped into five groups: a negative control (C-), a positive control immunized with toxoid (C+), and treatment groups T1, T2 and T3 immunized with toxoid and given daily oral administration of ethanolic Mimosa extract with concentrations of 1000, 500 and 250 mg/kg BW respectively for 14 days. Blood sample was taken and the obtained sera were tested with an Enzyme- Linked Immunosorbent Assay (ELISA) test. The results were analyzed using statistical ANOVA and Duncan Multiple Range Test. The conclusions of the research are Mimosa pudica extract can stimulate and increase the production of antibodies (Ig) in rats immunized with Naja sputatrix toxoid and the optimum dose for the treatment with Mimosa extract is 250 mg/kg BW