DOIAbstract
Summary: Ion Torrent sequencing is one of the most frequently used platforms in healthcare research and industry. Despite many advantages, platform-specific artifacts complicate efficient separation of true variants from errors, especially in variants with lower allele frequencies (<15%). Here, we developed a multi-step filtering toolbox AIRVF that works on flowgram, raw and mapped reads and called variants to reduce artifact-driven false variant calls. Tests on sequencing data of standard reference material showed up to approximately 98% reduction of false variants when combined to conventional public pipelines and approximately 48% to the in-house commercial solution, with a minimal loss of sensitivity. Availability and implementation: Th...
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