Effect of polishing method on surface roughness and bacterial adhesion of zirconia-porcelain veneer

The purpose of this study was to evaluate the effect of various polishing methods on surface roughness of zirconia-porcelain veneer and to correlate the findings with early bacterial adhesion. The study specimens were glazed (control group), glazed after fine polishing (glazed group) and polished with Exa Cerapol (Cerapol group) or with Shofu porcelain adjustment kit (Shofu group) (n =20). Surface roughness was then measured using profilometer and scanning electron microscope (SEM). After artificial saliva coating, the specimens were incubated in Streptococcus mitis suspension for 4 h at 37 °C. Adherent bacteria were quantified from SEM images. Streptococcal viability was assessed by LIVE/DEAD staining kit and fluorescent microscope. There were significant differences in surface roughness according to polishing method and surface material. Relatively smoother surfaces were found in zirconia surfaces and glazed porcelain surfaces. There were also significant differences in bacterial adhesion according to polishing method and surface material. Cerapol group showed minimal bacterial adhesion with more dead cells when compared to other groups. A positive correlation between surface roughness and bacterial adhesion was found in glazed porcelain surface and a negative correlation in zirconia surface of Cerapol group, both with no statistical significance. Within the limitations of in vitro study, surface roughness and bacteria adhesion were significantly influenced by polishing method and surface material. Also, there was a positive correlation and negative correlation between surface roughness and bacterial adhesion in glazed porcelain surface and in zirconia surface of Cerapol group, respectively.restrictio