CD4+CD45RBhigh T 면역세포를 처치한 면역억제 마우스에서 치조골 흡수 양상에 관한 연구

Dept. of Dental Science/박사Objectives: The aims of this study were to determine whether the antigen-inexperienced [naive, CD45RB high density] T-cell (CD4+CD45RBHigh T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell-transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals.Materials and Methods: CD4+CD45RBHigh and CD4+CD45RBLow [antigen-experienced (memory, CD45RB low density)] T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory-bowel-disease (IBD)-like syndrome (n = 8). hPDLSCs were transplanted into T-cell-transferred SCID mice to examine ectopic cementum formation 8 weeks after T cell transfer. The mandibles and tibias of these mice were retrieved for micro-computed tomography (micro-CT), histomorphometric analysis, and isolation of alveolar bone cells 16 weeks after T cell transfer. The in vitro osteogenic and adipogenic potentials of the ABCs were evaluated.Results: Histological and micro-CT analysis revealed that the transfer ofCD4+CD45RBHigh T-cell subset was sufficient for alveolar bone resorption and affected the osteogenic/adipogenic potential of ABCs. Furthermore, it was found that CD4+CD45RBHigh T cell-transferred animals have decreased systemic osteogenic potential, as evidenced using the in vivoectopic hPDLSC transplantation model.Conclusion: CD4+CD45RBHigh T-cell transfer induced both alveolar bone resorption and reduced systemic osteogenic potential, with a concomitant down regulation of the osteogenic potential of ABCs.ope