(The) experimental studies on amino acids imbalance of rice protein in rats

의학과/박사[한글] The Experimental Studies on Amino acids Imbalance of Rice Protein In Rats Doo Shik Choe Department of Biochemistry Graduate School, Yonsei University (Directed by Professor Chung Suk Song) Krehl et al.(1945) made an important discovery of the relationship of niacin and tryptophan in their experiments and these experiments extended to investigation of amino acids imbalance. The addition of DL-threonine of DL-phenylalanine to 9% casein sucrose diet produced amino acid imbalance which was reversed by the supplementation of niacin or tryptophan (Krehl et al., 1945, Hankes et al., 1948). The amino acids imbalance with rice diet was investigated by Pecora and Hundley(1951). They supplemented the rice diet with lysine and threonine which resulted in increasing the rate of gain of rats fed the supplemented rice diet. Though amino acids imbalance was investigated by many workers, much still remains unknown about the mechanism of the imbalance. The purpose of this study is to investigate the growth pattern and some of the biochemical change in rats fed a rice protein diet supplemented with various amino acids, and to study the mechanism of this imbalance. The body growth, various ascorbic acid content in blood and liver, and lipid content in the liver or rats, fed rice protein diet supplemented with lysine and threonine or blycine and methionine were investigated and the following results were obtained. 1. The body weight of rats fed a 705% rice protein diet supplemented with lysine and threonine showed less weight loss than that of the control group receiving 705% rice protein diet alone; and the rate of weight gain or rats fed with 10% or 15% rice protein diet supplemented with lysine and threonie was greater than that of the control group receiving 10% or 15% rice protein diet alone. 2. No difference was observed between the rate of weight gain in rats fed 705% rice pretein diet supplemented with glycine and methionine and that of rats receiving 705% rice protein diet alone. But the rate of weight gain of rats fed with 15% rice protein diet supplemented with glucine and methionine were greater than that of the control receiveing 15% protein diet alone. 3. No difference was observed between the vitamin C level in blood and liver of rats fed the diet suplemented with lysine and threonine, but vitamin C concentration in blood was increased as the content of rice protein in diet was raised. 4. The vitamin C in blood of rats fed the diet supplemented with glycine and methionine seemed to be little higher than that of the control fed the diet alone. But no difference was observed between the vitamin C level in liver of the experimental rats fed the diet supplemented with glycine and methionine and that of the control group fed the diet alone. [영문] Krehl et al.(1945) made an important discovery of the relationship of niacin and tryptophan in their experiments and these experiments extended to investigation of amino acids imbalance. The addition of DL-threonine of DL-phenylalanine to 9% casein sucrose diet produced amino acid imbalance which was reversed by the supplementation of niacin or tryptophan (Krehl et al., 1945, Hankes et al., 1948). The amino acids imbalance with rice diet was investigated by Pecora and Hundley(1951). They supplemented the rice diet with lysine and threonine which resulted in increasing the rate of gain of rats fed the supplemented rice diet. Though amino acids imbalance was investigated by many workers, much still remains unknown about the mechanism of the imbalance. The purpose of this study is to investigate the growth pattern and some of the biochemical change in rats fed a rice protein diet supplemented with various amino acids, and to study the mechanism of this imbalance. The body growth, various ascorbic acid content in blood and liver, and lipid content in the liver or rats, fed rice protein diet supplemented with lysine and threonine or blycine and methionine were investigated and the following results were obtained. 1. The body weight of rats fed a 705% rice protein diet supplemented with lysine and threonine showed less weight loss than that of the control group receiving 705% rice protein diet alone; and the rate of weight gain or rats fed with 10% or 15% rice protein diet supplemented with lysine and threonie was greater than that of the control group receiving 10% or 15% rice protein diet alone. 2. No difference was observed between the rate of weight gain in rats fed 705% rice pretein diet supplemented with glycine and methionine and that of rats receiving 705% rice protein diet alone. But the rate of weight gain of rats fed with 15% rice protein diet supplemented with glucine and methionine were greater than that of the control receiveing 15% protein diet alone. 3. No difference was observed between the vitamin C level in blood and liver of rats fed the diet suplemented with lysine and threonine, but vitamin C concentration in blood was increased as the content of rice protein in diet was raised. 4. The vitamin C in blood of rats fed the diet supplemented with glycine and methionine seemed to be little higher than that of the control fed the diet alone. But no difference was observed between the vitamin C level in liver of the experimental rats fed the diet supplemented with glycine and methionine and that of the control group fed the diet alone.restrictio