Clinical anti-halitosis effects of oral spray containing Curcuma xanthorrhiza oil

Objectives: The purpose of this study was to determine effects of anti-halitosis for oral spray containing Curcuma xanthorrhiza oil(Cx) by in-vitro and in-vivo method. Methods: This study used three kinds of oral sprays. First experimental group was containing 0.025% Curcuma xanthorrhiza oil plus 7% essential oil(CX+EO), second experimental group was containing 7% essential oil alone(EO), and control group was not containing any active ingredients. In-vitro trial, after mixing saliva from taking 10 subjects and adding these 20-200 ㎕ oral sprays, the levels of whole-mouth volatile sulphur compounds(VSCs) were measured by means of a gas chromatography(GC). In-vivo study, sixteen volunteers(7 male and 9 female), with a healthy oral status, were enrolled in a double-blind, cross-over design. After using above oral spray up to 3 hours, the levels of VSCs were measured by means of a GC and organoleptic analysis. Results: The effects of anti-halitosis by GC using salivary samples from taking volunteers were that CX+EO group showed higher effects of anti-halitosis than control group in proportion to dosage(p<0.05). In the result of clinical trial of effects of anti-halitosis for sixteen volunteers, CX+EO group has more effectiveness of anti-halitosis than EO group in GC evaluation from 1 to 2 hours and in organoleptic analysis at 3 hours(p