Study of the NPF and NRT transporter families in the conifer Pinus pinaster

Nitrogen, in natural environments, is the main limiting nutrient for plant growth and development1. This essential element can be presented in different forms in the soils being the two main forms nitrate and ammonium, although the relative abundance of these compounds depends on environmental conditions. In plants, nitrate uptake and transport involve the function of specific transporters, mainly of the NRT and NPF families. Genes encoding these transporters have been well studied in crop plants since in agricultural soils nitrate has become the predominant nitrogen compound as a result of the intensive use of fertilizers2. However, in soils of the large conifer forests dominating the boreal ecosystems, ammonium is the main source of inorganic nitrogen due to the limited nitrification process3. Consequently, conifers have been told to have a preference for ammonium over nitrate and only limited information is available about nitrate transporters in these gymnosperms. Maritime pine (Pinus pinaster Aiton) is a conifer tree with a wide distribution in the western Mediterranean region and with a great morphological and physiological plasticity. This pine also has a good resistance to abiotic stress4. In maritime pine, the genomic resources have been improved in the last few years5 allowing the identification and molecular analysis of members of the NRT and NPF gene families6. Growth and development responses have been compared on pine seedlings cultured under different nitrate and ammonium supplies. Gene expression have been analyzed and the results show a strong expression of different genes related with the uptake, transport and assimilation of nitrate in plants such as nitrate and nitrite reductases, glutamine synthetase and some genes from the NRT and NPF families, suggesting they are involved in nitrate acquisition from soil.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This project was supported by the grant MicroNUpE, BIO2015-73512-JIN; MINECO/AEI/FEDER, UE. JMVM was supported by a grant from the Spanish Ministerio de Educación y Formación Profesional (FPU17/03517) and FO by a grant from the Universidad de Málaga (Programa Operativo de Empleo Juvenil vía SNJG, UMAJI11, FEDER, FSE, Junta de Andalucía)