Map-based cloning of QFhb.mgb-2A identifies a WAK2 gene responsible for Fusarium Head Blight resistance in wheat

Fusarium graminearum is one of the most threating pathogen of wheat, responsible for Fusarium head blight (FHB) which annually leads to yield losses, grain quality decay and accumulation of harmful mycotoxins in kernels. Host resistance represents the most effective approach to limit disease damages; however, only a limited number of resistant loci have currently been detected in durum genotypes. In this work we report the map-based cloning of a FHB-QTL on 2A chromosome of durum wheat, introgressed from a resistant line derived from the Chinese wheat cv. Sumai-3. A marker enrichment of the QTL region was carried out leading to the inclusion of 27 new SNPs respect to the previous map. A wall-associated receptor-like kinase (WAK2) gene was identified in the region and sequenced, in the resistant parent ( R P) one gene was predicted accounting for a genomic sequence of 5,613 structured into 6 exons, whereas two adjacent genes were predicted on the same DNA plus strand of the susceptible parent ( S P).t The involvement of WAK2 gene in FHB resistance mechanism was assessed by gene expression comparison between resistant and susceptible wheat lines, and disease symptoms evaluation in 3 TILLING mutants for WAK protein function