Antiseptic efficacy of chlorhexidine and povidone iodine rinsing lingual mucous membrane in dogs

Problemi u usnoj šupljini pasa koji se trebaju kirurški tretirati su mnogobrojni. Poštivanje pravila i procedura dobre antisepse osobito je važno ako se uzme u obzir činjenica da u usnoj šupljini psa ima veliki broj bakterija koje čine sluznicu usta zagađenijom nego površinu kože. Zbog toga je jako važno odabrati antiseptik koji će biti doista učinkovit i koji će u velikom postotku smanjiti broj patogenih mikroorganizama, čime bi se smanjila učestalost postoperacijskih komplikacija, osobito postoperacijskih infekcija. Cilj našeg ispitivanja bio je istražiti osnovnu aerobnu bakterijsku mikrofloru u ustima pasa i istražiti antiseptičku učinkovitost 0,4% klorheksidin glukonata i 1% povidon-joda na sluznici jezika u pasa. U ispitivanje su bila uključena 45 pasa, podijeljena u tri skupine. Skupina KG je tretirana s 0,4% klorheksidin glukonatom, skupina PJ s 1% povidon-jodom i kontrolna skupina FO s 0,9% fiziološkom otopinom. Brisevi su uzimani s dorzalne strane jezika, prije i nakon tretmana s antisepticima ili s fiziološkom otopinom za kontrolnu skupinu. Broj bakterija smo određivali semikvantitativnom metodom, a identifikacija izraslih bakterijskih kolonija je vršena nakon precjepljivanja pojedinačnih kolonija na krvne agare. Od ukupno 90 uzetih briseva izolirali smo i identificirali 29 vrsta aerobnih bakterija. U pogledu antiseptičke učinkovitosti, i klorheksidin glukonat i povidon-jod su pokazali statistički značajnu redukciju rasta bakterijskih kolonija u usporedbi s kontrolnom skupinom. Nije bilo statistički značajne razlike između ova dva antiseptika u pogledu njihove učinkovitosti na redukciju rasta bakterijskih kolonija, a broj pozitivnih briseva koje smo dobili nakon ispiranja i s klorheksidin glukonatom i s povidon-jodom bio je identičan. Na temelju dobivenih rezultata može se zaključiti da je dvominutno ispiranje sluznice jezika s 0,4% klorheksidin glukonatom ili s 1% povidon-jodom preporučljivo za pripremu usta za kiruršku intervenciju.Oral disease in dogs occurs as frequently as in humans. There are many different oral diseases in dogs requiring surgical treatment. The hypothesis of this study was that the antiseptics chlorhexidine gluconate and povidone iodine inhibit bacterial growth and division on the surface of the lingual mucosa. The goal of the study was to examine the basic aerobic bacterial microflora in the mouths of dogs, and to test the antiseptic efficacy of 0.4% chlorhexidine gluconate (CG) and 1% povidone iodine on the lingual mucous membrane. A total of 45 dogs were included in the study, and divided into three groups. The CG group was treated with 0.4% chlorhexidine gluconate, the PI group was treated with 1% povidone iodine, and the control group S was treated with 0.9% saline. Samples were taken by swabbing the dorsal side of the tongue before and after treatment with the antiseptic or saline solution (n= 90 swabs). The number of bacteria was determined using the semi-quantitative method and identification of grown bacteria was performed following inoculation of separate colonies in blood agar. A total of 29 types of aerobic bacteria were isolated and identified from 90 swabs. In terms of efficacy, both chlorhexidine gluconate and povidone iodine showed a statistically significant reduction of the growth of bacterial colonies compared to the control group. There were no statistically significant differences between the two antiseptics in terms of their efficacy to reduce bacterial colonies, i.e. the same number of positive samples was obtained after rinsing with chlorhexidine gluconate and povidone iodine. It can be concluded that a 2-minute rinse of the lingual mucosa with 0.4% chlorhexidine gluconate or 1% povidone iodine is recommended as preparation of this part of the mouth for surgical intervention