Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation

Bacillus subtilis DprA and RecX proteins, which interact with RecA, are crucial for efficient chromosomal and plasmid transformation. We showed that RecA, in the rATP·Mg2+ bound form (RecA·ATP), could not compete with RecX, SsbA or SsbB for assembly onto single-stranded (ss)DNA, but RecA·dATP partially displaced these proteins from ssDNA. RecX promoted reversible depolymerization of preformed RecA·ATP filaments. The two-component DprA-SsbA mediator reversed the RecX negative effect on RecA filament extension, but not DprA or DprA and SsbB. In the presence of DprA-SsbA, RecX added prior to RecA·ATP inhibited DNA strand exchange, but this inhibition was reversed when RecX was added after RecA. We propose that RecA nucleation is more sensitive to RecX action than is RecA filament growth. DprA-SsbA facilitates formation of an active RecA filament that directly antagonizes the inhibitory effects of RecX. RecX and DprA enable chromosomal transformation by altering RecA filament dynamics. DprA-SsbA and RecX proteins constitute a new regulatory network of RecA function. DprA-SsbA contributes to the formation of an active RecA filament and directly antagonizes the inhibitory effects of RecX during natural transformation.Spanish Ministerio de Economía y Competitividad (MINECO) Predoctoral Fellowship [BES-2013–063433 to E.S.]; MINECO/FEDER Grant [BFU2015–67065-P to J.C.A., in part]; Singapore Ministry of Education Academic Research Fund Tier 3 [MOE2012-T3–1-001 to J.Y., in part]; National Research Foundation through the Mechanobiology Institute Singapore (to J.Y.) (in part); National Research Foundation, Prime Minister’s Office, Singapore under its NRF Investigatorship Programme [Award NRFNRFI2016–03 to J.Y., in part]. Funding for open access charge: MINECO/FEDER [BFU2015–67065-P].Peer reviewe