Metabolite profiling of ethyl acetate extract from Marsilea crenata Presl. Using UPLC-QToF-MS/MS

Marsilea crenata Presl. is a plant that widely used as traditional food in Surabaya, Indonesia. Although in some research it was known contain phytoestrogens which have activity in bone formation, the phytochemical properties of M. crenata has not been completely confirmed yet. The aim of this research was to determine the metabolite profile of ethyl acetate extract of M. crenata using UPLC-QToF-MS/MS, which can be used as a reference for further research and utilization of M. crenata. Dried powder of M. crenata was extracted with n-hexane followed by ethyl acetate. The 100 ppm of ethyl acetate extract in DCM and methanol then injected 5 µl each into the UPLC-QToF-MS/MS. The results were analyzed by Masslynx 4.1 software, and showed various types of compounds, either detected compounds (36 compounds), or unknown compounds