We report dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR spectroscopy in viral capsids from HIV-1 and bacteriophage AP205. Viruses regulate their life cycles and infectivity through modulation of their structures and dynamics. While static structures of capsids from several viruses are now accessible with near-atomic-level resolution, atomic-level understanding of functionally important motions in assembled capsids is lacking. We observed up to 64-fold signal enhancements by DNP, which permitted in-depth analysis of these assemblies. For the HIV-1 CA assemblies, a remarkably high spectral resolution in the 3D and 2D heteronuclear data sets permitted the assignment of a significant fraction of backbone and side-ch...
International audienceDynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivi...
Polenova, TatyanaA key step in the HIV-1 lifecycle is viral maturation, which proceeds through the p...
SummaryMature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and p...
We report dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR spectroscopy in...
We report remarkably high, up to 100-fold, signal enhancements in 19F dynamic nuclear polarization (...
Polenova, TatyanaHuman immunodeficiency virus (HIV) is the causative agent for the acquired immune d...
HIV-1 CA capsid protein possesses intrinsic conformational flexibility, which is essential for its a...
The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously sel...
The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously sel...
International audienceSolid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables t...
Structural virology reveals the architecture underlying infection. While notably electron microscopy...
International audienceStructure determination of adjuvant-coupled antigens is essential for r...
While solid-state nuclear magnetic resonance (ssNMR) has emerged as a powerful technique for studyin...
The retroviral capsid protein (CA) is derived from the cleavage of Gag polyprotein during the matura...
International audienceDynamic Nuclear Polarization (DNP) is an effective approach to alleviate the i...
International audienceDynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivi...
Polenova, TatyanaA key step in the HIV-1 lifecycle is viral maturation, which proceeds through the p...
SummaryMature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and p...
We report dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR spectroscopy in...
We report remarkably high, up to 100-fold, signal enhancements in 19F dynamic nuclear polarization (...
Polenova, TatyanaHuman immunodeficiency virus (HIV) is the causative agent for the acquired immune d...
HIV-1 CA capsid protein possesses intrinsic conformational flexibility, which is essential for its a...
The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously sel...
The 231-residue capsid (CA) protein of human immunodeficiency virus type 1 (HIV-1) spontaneously sel...
International audienceSolid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables t...
Structural virology reveals the architecture underlying infection. While notably electron microscopy...
International audienceStructure determination of adjuvant-coupled antigens is essential for r...
While solid-state nuclear magnetic resonance (ssNMR) has emerged as a powerful technique for studyin...
The retroviral capsid protein (CA) is derived from the cleavage of Gag polyprotein during the matura...
International audienceDynamic Nuclear Polarization (DNP) is an effective approach to alleviate the i...
International audienceDynamic nuclear polarization (DNP) is a powerful way to overcome the sensitivi...
Polenova, TatyanaA key step in the HIV-1 lifecycle is viral maturation, which proceeds through the p...
SummaryMature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and p...