Corazon Genome Annotation Project

Corazon Genome Annotation Project Mycobacterium phage, Corazon, was found at Lafayette College in Easton, Pennsylvania in 2017. Its plaque was small, round and clear with siphoviridae morphology type. The approximate length of Corazon is 64931 bp with 3’ sticky overhang. The overhang had 11 base length with ‘GCGCGCAGCGC’ sequence. To perform a manual inspection of Corazon, we performed gene annotation by reviewing and revising the prediction and identifying any missing genes using DNA Annotation programs such as DNA Master, GeneMark, BLAST, Phamerator, and HHPred. We annotated the Corazon genome in three distinct steps. First, we established a relationship with our phage, Corazon, and other phages to understand the overall genomic architectures. Second, we ran automated gene prediction proteins and functional data on the predicted gene. Third, we reviewed the prediction and made necessary changes to delete or identify any missing genes. The group annotated the mycobacterium Corazon genes 33-48. Based on the evidence supported by the programs, most of the Corazon genes from 33 to 48 were forward genes with functions such as terminase, portal protein, capsid maturation protease, scaffolding protein, head decorate protein, major capsid protein, portal protein, major tail protein, and tail assembly chaperone. Tail assembly chaperones are only shown in frameshift genes, in this case, Gene 47. Gene 47 is a frameshift gene which a nucleotide reads more than once or omitted to have two genes in the same space. Since Gene 47 is a frameshift gene, Gene 46 and 47 are overlapping. Based on the predicted functions, the segment of genes 33 to 48 of Corazon mainly consists of structural genes that are largely responsible for the formation of the phage structure. By annotating the gene in this project, it will determine the known functions of the newly discovered phage, and it will contribute to the exploration of phage genomes. Keywords: Corazon, DNA Annotation, DNA Master, Genemark, gene, portal protein, major tail protein, tail assembly chaperone, capsid maturation protease