Habitat use of important juvenile fish species in temperate Earnestine nursery areas with notes on feeding ecology and historical shifts to assist with conservation planning, South Africa

Estuaries are among the most productive ecosystems worldwide as they provide important refuge and feeding areas for the juveniles of many marine species, many of which have recreational importance. The use of these nursery areas by juvenile fishes in South Africa are in need of further investigation and many critical areas such as shallow water creeks and vegetated habitats remain understudied. By using mixed-methods, estuarine fish assemblages were assessed in five permanently open estuaries in the Eastern Cape Province of South Africa. A total of 81 428 comprising 24 families and 40 species of fishes were sampled by means of a 50 m seine net (12 mm mesh) along the length of these systems that comprised of six equidistant sites during the summer recruitment period (October - December) of 2014 and 2015. A higher catch per unit effort (CPUE) was recorded in 2014 (68 875) compared to the following year where a decline of approximately 82% was observed in the catch (12 553) for all estuaries except the Kromme Estuary. Catches were mainly dominated by the marine estuarine dependent sparid, Rhabdosargus holubi and the solely estuarine clupeid, Gilchristella aestuaria. Species compositions and catch abundance with regards to historical catches showed that the abundance of many marine estuarine dependent juveniles as well as important recreational species have declined within these systems and are mainly attributed to marine overfishing and the exploitation of undersized individuals in estuarine nurseries. Juvenile fishes that occur in these estuaries utilize a range of vegetated habitats ranging from salt marsh creeks, Zostera capensis beds, Spartina maritima and previously unexplored Phragmites australis areas. Sampling was achieved by using passive, double-winged, six-hooped fyke nets, 1 mm mesh size that was secured in placed on the nocturnal flood tide at each inundated vegetated and adjacent unvegetated habitat types. Higher catches were frequently recorded in vegetated areas for solely estuarine and marine estuarine dependent species and supports evidence of plasticity within these habitats. In general, the previously unstudied reed, P. australis showed the highest species richness and abundance of juvenile fishes overall, followed by, Z. capensis and the intertidal salt marsh species, S. maritima. These findings relate to many international trends on the value of vegetated areas as refugia for young fishes in estuaries. Fishes were also showing evidence of feeding and sheltering in these areas. By using conventional stomach contents and stable isotope methods (δ13C and δ15N signatures) on the abundant R. holubi in previously selected vegetation sites, it was clear that this species has a significant reliance on any of these habitats as their diets was largely comprised of a mixture of epiphytic algae and aquatic macrophytes. In addition, the diet of larger individuals (>80 mm) showed a greater component of invertebrates in previously unexplored habitats, which proves their omnivorous diet related to ontogenetic niche use. Knowledge of fine-scale habitat use by fishes is essential for conservation planning as exclusion zones can be optimised to limit anthropogenic influence on nursery use patterns in fishes. The availability of shallow water habitats (