Preparation of biodegradable nanoparticles for hydrophilic macromolecular drugs delivery

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Ondřej Holas, PhD. Student: Alexandra Szanyiová Title of thesis: Preparation of biodegradable nanoparticles for hydrophilic macromolecular drugs delivery This study investigates the formulation of nanoparticles containing hydrophilic macromolecular components (e.g. proteins). Selected material was PLGA based compounds synthesized at Department of Pharmaceutical Technology. As model compounds were used Rhodamine B, FITC labelled dextran and FITC labelled albumin. Selected methods of nanoparticles formulation were double-emulsion technique and nanoprecipitation. Prepared nanoparticles were purified by three cycles of centrifugation and encapsulation efficacy and recovery yield was measured. Effect of different polymers and stabilizers was followed. More specifically, the principal objective was to explore the differences between size, zeta potential and efficacy of encapsulation. Changes in these characteristics were brought about by the chosen polymers, stabilizers, encapsulated compound, length of centrifugation period. Prepared nanoparticles had size ranging between 150-474 nm and zeta potential approximately 30 mV. Even though the main goal of the study was to..