Voltammetric Techniques for Analysis in a Single Drop of a Solution

This contribution describes miniaturization of voltammetric methods and some of the main problems caused by reducing the sample volume to 20 mu L. This study was carried out in dimethyl sulfoxide solutions and buffered aqueous solutions with 10% DMSO at a glassy carbon electrode. A novel antibiotic agent, 1-hydroxy-N-(4-nitrophenyl) naphthalene-2-carboxamide, was used as a model substance. This analyte was determined by cathodic and anodic voltammetry. Elimination of the negative influence of dissolved oxygen was performed in various manners. Two most effective methods were square wave voltammetry that can be used in the presence of dissolved oxygen and removal of oxygen in a microcell with nitrogen atmosphere inside