Fast speciation analysis of selenoproteins in a micro-drop of human serum by microbore affinity-HPLC hyphenated to ICP-sector field-MS

For the rst time a new ecient analytical approach based on microbore AF-HPLC hyphenated to ICP-sector eld MS is proposed for the simultaneous speciation analysis of the selenoproteins glutathione peroxidase, selenoprotein P and seleno-albumin in human serum without prior sam- ple preparation or mathematical correction of the spectral interferences. Microbore AF-HPLC allows the reduction of sample volume to a single micro-drop (5 L) and the decrease of chro- matographic run time to 7 min. A high-eciency sample introduction system is also proposed to achieve an enhancement in sensitivity of more than one order of magnitude