Add to ORKGAnion exchange-solid-phase extraction carried out prior to double column affinity-high performance liquid chromatography (AF-HPLC) coupled on-line with conventional inductively coupled plasma-(quadrupole) mass spectrometry is proposed for simultaneous speciation analysis of selenoprotein P (SelP), glutathione peroxidase (GPx) and selenoalbumin (SeAlb) in human serum. Efficient inteference-free free AF-HPLC separation of GPx, SelP and SeAlb is obtained within a total chromatographic run time less than 15 min. The repeatability in terms of relative standard deviation (R.S.D., %, n = 10) is 7% for GPx and SelP and 4% for SeAlb. The detection limits are 0.1 ng mL(-1) for GPx, 1.0 ng mL(-1) for SelP and 1.3 ng mL(-1) for SeAlb. The method was va...
Se is one of the most investigated essential trace elements in the past years, mostly due to its can...
Se is one of the most investigated trace essential elements in the last years, mostly due to its can...
Several recent analytical methods for determination of Se and selenoprotein P have involved high-per...
Anion exchange-solid-phase extraction carried out prior to double column affinity-high performance l...
A method based on anion exchange (AE) and affinity (AF)-HPLC (AE-AF-HPLC) hyphenated to inductively...
This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid...
For the rst time a new ecient analytical approach based on microbore AF-HPLC hyphenated to ICP-sect...
The role of metallo-proteins in the body and their speciation is of increasing interest in clinical ...
A method for the simultaneous speciation of selenoproteins and selenometabolites in mouse plasma has...
Se is one of the most investigated essential trace elements in the past years, mostly due to its can...
Se is one of the most investigated trace essential elements in the last years, mostly due to its can...
Several recent analytical methods for determination of Se and selenoprotein P have involved high-per...
Anion exchange-solid-phase extraction carried out prior to double column affinity-high performance l...
A method based on anion exchange (AE) and affinity (AF)-HPLC (AE-AF-HPLC) hyphenated to inductively...
This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid...
For the rst time a new ecient analytical approach based on microbore AF-HPLC hyphenated to ICP-sect...
The role of metallo-proteins in the body and their speciation is of increasing interest in clinical ...
A method for the simultaneous speciation of selenoproteins and selenometabolites in mouse plasma has...
Se is one of the most investigated essential trace elements in the past years, mostly due to its can...
Se is one of the most investigated trace essential elements in the last years, mostly due to its can...
Several recent analytical methods for determination of Se and selenoprotein P have involved high-per...