Add to ORKGDroplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of transcriptomes. However, assessing differential expression across multiple individuals has been hampered by inefficient sample processing and technical batch effects. Here we describe a computational tool, demuxlet, that harnesses natural genetic variation to determine the sample identity of each droplet containing a single cell (singlet) and detect droplets containing two cells (doublets). These capabilities enable multiplexed dscRNA-seq experiments in which cells from unrelated individuals are pooled and captured at higher throughput than in standard workflows. Using simulated data, we show that 50 single-nucleotide polymorphisms (SNPs) per ...
For the past several decades, due to technical limitations, the field of transcriptomics has focused...
The development of single cell transcriptome sequencing has allowed researchers the possibility to d...
Funder: Medical Research CouncilMultiplexed single-cell RNA-seq analysis of multiple samples using p...
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of t...
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of t...
Computational approaches offer substantial ability to improve annotation and interpretation of a ran...
A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequenci...
A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequenci...
Methods to deconvolve single-cell RNA-sequencing (scRNA-seq) data are necessary for samples containi...
Abstract Background Here we present scSNPdemux, a sample demultiplexing pipeline for single-cell RNA...
Background: Recent advancements in Next Generation Sequencing (NGS) and high throughput multiplexed ...
SummaryIt has long been the dream of biologists to map gene expression at the single-cell level. Wit...
One powerfull tool in understanding how genetic variations can lead to changes in gene expression ac...
With the improvement of sequencing protocols and decreasing sequencing costs, single-cell RNA seque...
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cel...
For the past several decades, due to technical limitations, the field of transcriptomics has focused...
The development of single cell transcriptome sequencing has allowed researchers the possibility to d...
Funder: Medical Research CouncilMultiplexed single-cell RNA-seq analysis of multiple samples using p...
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of t...
Droplet single-cell RNA-sequencing (dscRNA-seq) has enabled rapid, massively parallel profiling of t...
Computational approaches offer substantial ability to improve annotation and interpretation of a ran...
A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequenci...
A variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequenci...
Methods to deconvolve single-cell RNA-sequencing (scRNA-seq) data are necessary for samples containi...
Abstract Background Here we present scSNPdemux, a sample demultiplexing pipeline for single-cell RNA...
Background: Recent advancements in Next Generation Sequencing (NGS) and high throughput multiplexed ...
SummaryIt has long been the dream of biologists to map gene expression at the single-cell level. Wit...
One powerfull tool in understanding how genetic variations can lead to changes in gene expression ac...
With the improvement of sequencing protocols and decreasing sequencing costs, single-cell RNA seque...
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cel...
For the past several decades, due to technical limitations, the field of transcriptomics has focused...
The development of single cell transcriptome sequencing has allowed researchers the possibility to d...
Funder: Medical Research CouncilMultiplexed single-cell RNA-seq analysis of multiple samples using p...