The Distribution of Alcohol in the Tissues of the Rat

In order to determine the distribution of alcohol in the tissues, we have devised a method which is quite simple and accurate. The animal is killed and the tissues removed as rapidly as possible. The tissues are placed in weighed 150 ml. extraction flasks immersed in solid carbon dioxide. The flasks and tissues are weighed and the tissues covered with a solution of picric and tartaric acids. The samples are then placed in a refrigerator until the analysis can be made