Culture of Excised Embryos of Pinus Ponderosa

Embryos of Pinus ponderosa Laws. were excised and placed on a nutrient agar medium to which auxins in varying concentrations had been added. Results indicated that when the auxins were dissolved in and diluted with 40 per cent alcohol there was inhibition of seedling growth and development. Techniques were devised which eliminated the use of alcohol. In the absence of alcohol, morphologically normal seedlings were produced which were transferred to quartz sand after 60 days and at 113 days were transplanted into soil. Apparent normal growth and development continued throughout the duration of the experiment. The addition of auxins produced no significant stimulation to the growth and development of the seedlings