Protective Effects of Pretreatment with Ginsenosides on Cardiac and Coronary Vascular Function After Hypothermic Rat Heart Preservation

Prevention of cardiac and vascular dysfunction with pretreatment has been accepted as an important factor in heart transplantation. Ginsenosides (GS) have been reported to have some beneficial effects on the cardiac and vascular system. We hypothesized that pretreatment with GS would result in an improvement of functional recovery after a 12 hour (hr) rat heart preservation. A Langendorff apparatus was applied to estimate the cardiac and vascular function in an isolated rat heart preparation. The hearts were preserved in University of Wisconsin solution at 0°C for 12hr, after pretreatment with 0.9% sodium chloride or GS 100 mg/kg, respectively, in control (n = 9) and GS (n = 14) groups. After storage, the cardiac function, myocardial water content, and coronary vasodilatory response were evaluated. The GS group showed a significantly higher recovery percentage of cardiac function compared with the control group: aortic flow 81.4 ± 21.4% versus 57.2 ± 11.0% (p = 0.0052); coronary flow 81.4 ± 14.5% versus 57.2 ± 6.0% (p = 0.0001); ±dp/dt max 72.5 ± 16.1 % and 66.0 ± 16.1 % versus 53.7 ± 4.1 % and 51.4 ± 7.1 % (p = 0.0027 and p = 0.0189) respectively. The GS group showed a lower increase in myocardial water content. With Langendorff perfusion, the endothelial and vascular smooth-muscle cell function were evaluated by an increasing percentage of coronary flow in response to acetylcholine chloride (0.3 × 10-7 mol/liter) and nitroglycerin (0.5 × 10-5 mol/liter). It was significantly higher in the GS group than that in the control group (19.2 ± 8.8% and 28.0 ± 14.1% versus 9.9 ± 4.7% and 14.7 ± 8.1%, p = 0.008 and p = 0.0187, respectively) at the first minute. These results suggest a protective effect on ventricular and coronary vascular function in the rats pretreated with Ginsenosides, indicating potential benefits for long-term heart preservation