Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes , France

Moura, Alexandra,
Tourdjman, Mathieu
Leclercq, Alexandre
Hamelin, Estelle
Laurent, Edith
Fredriksen, Nathalie
van Cauteren, Dieter
Bracq-Dieye, Hélène
Thouvenot, Pierre
Vales, Guillaume
Tessaud-Rita, Nathalie
Maury, Mylène,
Alexandru, Andreea
Criscuolo, Alexis
Quevillon, Emmanuel
Donguy, Marie-Pierre
Enouf, Vincent
De Valk, Henriette,
Brisse, Sylvain
Lecuit, Marc

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Publication date

September 2017

DOI

10.3201/eid2309.170336

Publisher

Centers for Disease Control and Prevention (CDC)

Language

English

Abstract

International audienceDuring 2015-2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p<0.001). cgMLST discriminated unrelated isolates that shared identical PFGE profiles and phyloge...