The paper investigates the field of Entrepreneurial Ecosystem's Governance in order to evaluate the relation between entrepreneurial ecosystem and governance and to define a framework able to launch, develop and sustain the ecosystem over time. From an analysis on heterogeneous sample of people linked to start-ups and spin-offs of University of Salento, several issues of entrepreneurial context are emerged. The framework proposed, leveraging on triple helix approach, foresees work's paths in which three helices acting synergistically and hold the operational procedures coming from four models of business ecosystem. Additionally, we take into account the business environment's dynamicity, encompassing evolutionary paths able to support the b...