Quantification of freely dissolved effect concentrations in in vitro cell-based bioassays

Improved understanding of chemical exposure in in vitro bioassays\ua0is required for quantitative in vitro-in vivo extrapolation (QIVIVE). In this study, we quantified freely dissolved concentrations in medium sampled from in vitro cell-based bioassays (C) for nine chemicals with different hydrophobicity and speciation at the time point of dosing and after an incubation period of 24\ua0h using solid-phase microextraction. The chemicals were tested in two reporter gene assays, the AREc32 assay indicative of the oxidative stress response and the PPARγ-GeneBLAzer assay that responds to chemicals which bind to the peroxisome proliferator-activated receptor\ua0gamma. For seven of the nine chemicals, C did not change significantly over time in both assays and the experimentally determined C generally agreed well with predictions of a mass balance model that describes the partitioning between proteinaceous and lipidous medium constituents, cells and the aqueous phase. Two chemicals showed a decrease of C in the AREc32 assay over time that was probably caused by cellular metabolism. Furthermore, C of the acidic chemical diclofenac deviated from the model predictions by more than a factor of 10 at higher concentrations, which indicates nonlinear binding and saturation of the medium proteins. Bioassay results are typically reported as nominal effect concentrations (EC), although it is established that freely dissolved effect concentrations (EC) are a better measure for the bioavailable dose and the method developed here provides a simple experimental approach to measure and model EC in in vitro bioassay for improved QIVIVE models