In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

International audienceThis study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared Within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised hos