Non-invasive assessment of lung pathobiology in hairdressers [Valutazione non invasiva della patobiologia polmonare in una coorte di lavoratori del settore acconciatura]

Background: Hairdressers are exposed to che mical agents with potentially irritant and sensitizing effects on airways. Objectives: To asses the presence of respiratory symptoms and biochemical and functional changes of the airways in a cohort of hairdressers. Methods: Respiratory symptoms, lung function tests, fractional exhaled nitric oxide (FENO50) and selected oxidative stress biomarkers [hydrogen peroxide (H2O2), malondialdehyde (MDA) and 4-hydroxynonenal (HNE)] in exhaled breath condensate (EBC) were assessed in 23 hairdressers on a rest day (Group 1); 12 workers (Group 2) were willing to perform the same tests at the beginning (BS) and at the end of a shift (ES) on the same working day. Eighteen subjects not occupationally exposed to airways irritants formed the control group. Results: Most of the hairdressers reported respiratory symptoms during work; however, all (except one) showed normal spirometry indexes. FENO50 levels were within the reference limits and did not change comparing BS vs. ES sampling. H2O2 and HNE values in EBC were higher in Group 1 (H 2O2: 0,16 [0,05-0,19] μM; HNE: 0,94 [0,82-1,22] nM) than in controls (H2O2: 0,05 [0,02-0,09] μM; HNE: 0,61 [0,49-0,78] nM, p<0,001). In Group 2, H2O2 and MDA levels were higher in EBC collected at ES (0,56 [0,23-3,62] mM and 5,21 [4,93-5,95] nM) in comparison with the BS values (0,11 [0,03-0,28] mM and 4,12 [3,46-5,16] nM, p<0,001 and p