The alanine:glyoxylate aminotransferase (AGT), a hepatocyte-specific pyridoxal-5'-phosphate (PLP) dependent enzyme, transaminates L-alanine and glyoxylate to glycine and pyruvate, thus detoxifying glyoxylate and preventing pathological oxalate precipitation in tissues. In the widely accepted catalytic mechanism of the aminotransferase family, the lysine binding to PLP acts as a catalyst in the stepwise 1,3-proton transfer, interconverting the external aldimine to ketimine. This step requires protonation by a conserved aspartate of the pyridine nitrogen of PLP to enhance its ability to stabilize the carbanionic intermediate. The aspartate residue is also responsible for a significant geometrical distortion of the internal aldimine, crucial f...
<p>Catalytically important amino acids and the cofactor of alanine transaminases: AlaA (<b>A</b>), <...
<div><p>Aspartate aminotransferase from <i>Corynebacterium glutamicum</i> (<i>Cg</i>AspAT) is a PLP-...
Abstract: Density functional methods have been applied to investigate the irreversible transaminatio...
Aspartate aminotransferase (AAT) is a prototypical pyridoxal 5'-phosphate (PLP) dependent enzyme tha...
The substitution of Ser187, a residue located far from the active site of human liver peroxisomal al...
Alanine:glyoxylate aminotransferase (AGT) is a pyridoxal-phosphate (PLP)-dependent enzyme. Its defic...
His-tagging is commonly used to aid and expedite the purification of recombinant proteins. It is com...
Human hepatic peroxisomal AGT (alanine:glyoxylate aminotransferase) is a PLP (pyridoxal 5'-phosphate...
Serine hydroxymethyltransferase belongs to the α class of pyridoxal-5′-phosphate enzymes along with ...
Human liver peroxisomal alanine:glyoxylate aminotransferase (AGT) is a pyridoxal 5'-phosphate (PLP)-...
Photorespiration is an energetically costly metabolic pathway for the recycling of phosphoglycolate ...
Human liver peroxisomal alanine:glyoxylate aminotransferase (AGT) is a pyridoxal 5'-phosphate (PLP)-...
Peroxisomal alanine:glyoxylate aminotransferase (AGT) is responsible for glyoxylate detoxification i...
16 pags, 5 figsAlanine-glyoxylate aminotransferase catalyzes the transamination between L-alanine an...
Primary Hyperoxaluria Type 1 (PH1) is a rare autosomal recessive disorder characterized by the depos...
<p>Catalytically important amino acids and the cofactor of alanine transaminases: AlaA (<b>A</b>), <...
<div><p>Aspartate aminotransferase from <i>Corynebacterium glutamicum</i> (<i>Cg</i>AspAT) is a PLP-...
Abstract: Density functional methods have been applied to investigate the irreversible transaminatio...
Aspartate aminotransferase (AAT) is a prototypical pyridoxal 5'-phosphate (PLP) dependent enzyme tha...
The substitution of Ser187, a residue located far from the active site of human liver peroxisomal al...
Alanine:glyoxylate aminotransferase (AGT) is a pyridoxal-phosphate (PLP)-dependent enzyme. Its defic...
His-tagging is commonly used to aid and expedite the purification of recombinant proteins. It is com...
Human hepatic peroxisomal AGT (alanine:glyoxylate aminotransferase) is a PLP (pyridoxal 5'-phosphate...
Serine hydroxymethyltransferase belongs to the α class of pyridoxal-5′-phosphate enzymes along with ...
Human liver peroxisomal alanine:glyoxylate aminotransferase (AGT) is a pyridoxal 5'-phosphate (PLP)-...
Photorespiration is an energetically costly metabolic pathway for the recycling of phosphoglycolate ...
Human liver peroxisomal alanine:glyoxylate aminotransferase (AGT) is a pyridoxal 5'-phosphate (PLP)-...
Peroxisomal alanine:glyoxylate aminotransferase (AGT) is responsible for glyoxylate detoxification i...
16 pags, 5 figsAlanine-glyoxylate aminotransferase catalyzes the transamination between L-alanine an...
Primary Hyperoxaluria Type 1 (PH1) is a rare autosomal recessive disorder characterized by the depos...
<p>Catalytically important amino acids and the cofactor of alanine transaminases: AlaA (<b>A</b>), <...
<div><p>Aspartate aminotransferase from <i>Corynebacterium glutamicum</i> (<i>Cg</i>AspAT) is a PLP-...
Abstract: Density functional methods have been applied to investigate the irreversible transaminatio...