Diversity of carbapenemases in clinical isolates of Enterobacteriaceae in Croatia-the results of a multicentre study

Since the first carbapenem-resistant Klebsiella pneumoniae strain was isolated in 2008, Enterobacteriaceae with reduced susceptibility to one or more carbapenems have emerged sporadically in different geographical regions in Croatia. These observations gave rise to a multicenter study on carbapenem resistance in Enterobacteriaceae from Croatia. Fifty-seven carbapenem-non-susceptible strains of Enterobacteriaceae were collected during 2011-2012 from four large hospital centres in Croatia. Overall, 36 strains produced VIM-1 -lactamase, three produced NDM-1, and one produced KPC-2. A high degree of clonal relatedness was observed in Enterobacter cloacae and Citrobacter freundii strains, in contrast to K.pneumoniae strains. Bla(VIM) genes were located within class1 integron which contained genes encoding resistance to aminoglycosides (aac(A4)). The study found strong association between bla(VIM) and qnr(B6) and between bla(NDM) and qnr(A6) genes