Nowadays, anaerobic digestion is a proven technology for the biowaste management. Current research interest is orientated towards process optimization in terms of biogas yield focusing amongst other on mechanical pre-treatment applications to favour solubilisation of biowaste complex organic compounds. A recent mechanical pre-treatment technique involves pressing of segregated biowaste for the physical separation of the material to distinct and more easily handled phases namely semi-liquid and semi-solid biowaste fractions. The aim of the current study is to examine the characteristics of the semi-liquid fraction and to assess the anaerobic digestion process under mesophilic (37 °C) and thermophilic (55 °C) conditions in CSTR pilot scale re...