Key role of dual specificity kinase TTK in proliferation and survival of pancreatic cancer cells
- Kaistha BP
- Honstein T
- Müller V
- Bielak S
- Sauer M
- Kreider R
- Schmees C
- Volkmer H
- Gress TM
- Buchholz M.
- Fassan, Matteo
- SCARPA, Aldo
DOIAbstract
Background:Pancreatic ductal adenocarcinoma (PDAC) is among the most aggressive human malignancies with an overall 5-year survival rate of <5%. Despite significant advances in treatment of the disease during the past decade, the median survival rate (∼6 months) has hardly improved, warranting the need to identify novel targets for therapeutic approaches.Methods:Quantitative real time PCR, western blot analyses and immunohistochemical staining of tissue microarrays were used to analyse the expression of TTK gene in primary PDAC tissues and cell lines. To inhibit TTK kinase expression in a variety of pancreatic cancer cell lines, RNA interference was used. Functional roles of this kinase in the context of PDAC were studied using cell prolifer...
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