Synthesis of a Superparamagnetic Iron Oxide Based Nano-complex for Targeted Cell Death of Glioblastoma Cells

In the last ten years, there has been little advancement in the treatment of Glioblastoma Multiforme (GBM). This research describes the synthesis of a superparamagnetic iron oxide (SPION)-based nanotheraputic complex for use in targeting and killing aggressive GBM cells. The properties of the synthesized SPIONs were precisely tailored via a novel time-controlled approach utilizing a previously described electrochemical reaction. Through magnetic and thermogenic analysis, Through this synthetic method, the optimal particle size (OPS) where maximal thermal energy is released was determined to be 21 nm. The nano-complex was further modified to selectively target GBM cells by adding a heterobifunctional poly(ethylene) glycol polymer cross-linked to TWEAK (a GBM targeting ligand). Further investigation with FITC Annexin V/Propidium Iodide fluorescent probe revealed that cells treated with the synthesized nano-complex showed markers positive for cellular apoptosis. Thus, these nano-complexes hold promise as a potential treatment agent for an otherwise untreatable disease