How Sequence Directs Structure: My First Steps to Unlock the Protein Folding Code in GB1

The β1 domain of Streptococcal protein G consists of 56 amino acid residues arranged in a two-layer alpha-beta sandwich. We are interested in understanding how the sequence encodes the three-dimensional structure of this protein. In support of this research investigation, two mutations were selected, designed and synthesized. The first is glutamic acid 42 to glycine and the second is valine 39 to alanine. The variant proteins were expressed in preparation for future experimental studies which will involve purifying the proteins as well as conducting thermodynamic and kinetic studies to elucidate the effect of the mutations on structure, stability and folding